Hi Himanshi
Generally glassware is baked in oven for RNA work...and it should be fine
bye
There will not be any problem if you autoclave it, then bake it, what do you mean by (will it affect the RNA quality? ) Best
Baking the glassware at about 200C is sufficient to destroy RNase activity- RNase was one of the first proteins to be studied in detail, and it was found that if a solution of RNase was boiled then cooled slowly, it refolded, and retained much of its activity. Indeed, if you make DNase free RNase, you boil it then cool it to destroy the DNase, but not the RNase. RNase cannot withstand a good anhydrous baking though! If you use Schott bottles, rinse the plastic caps in DEPC water then autoclave, as they will melt in the oven!
DEPC is used as a chemical inhibitor of RNase as it reacts with histidine residues in the protein. Add your DEPC to water, and leave it a while-I often leave it overnight at 37C, and then autoclave it twice, as any residue can react with sensitive downstream applications.
I autoclaved the glasswares after being treated by DEPC overnight. You could also bake the glasswares at 180C (or some would suggest 200C) before using with your RNA works. All buffers to be used should be DEPC treated overnight and autoclaved.
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