Can I use G418 for selection of HEK 293 cell lines that already have a plasmid with kan and Hygro integrated?

I am doing a kill curve in HEK 293 cells with G418(0-1000ug/mL) and they don't die. They were previously transfected and selected with hygromycin and are integrated with a plasmid has also...

05 December 2020 3,232 3 View

Adaptability of frugal health care systems into developed markets - can someone share ideas on methodology for dissertation?

Hello! Hope everyone is doing alright during these turbulent and uncertain times. I am working on my masters dissertation (MSc International Management) and would like to answer the research...

05 August 2020 6,155 4 View

What is the best production evaluation method in an architectural design company?

I would like to know what is the most effective method to manage processes in an architecture company

29 April 2020 2,932 1 View

Dissertation topic for MSc International Management?

Hello, I am doing my masters in international management and looking for a research question related to start-up/ authenticity/ crowdfunding/ entrepreneurship & innovation to do my dissertation...

21 April 2020 5,222 23 View

What are the evidences of the AI's impact on the company's scalability?

How could we differentiate a usual scalability (organic or without AI technologies application) from an AI-impacted scalability of the enterprise?

27 August 2019 3,081 4 View

Plasmatic proteins solvent?

Can anyone suggest me a solvent to solubilize plasmatic proteins? I tried with formic acid, but was parcially disolved. Thanks

03 July 2019 10,030 2 View

How can I induce sickling consistently with sodium metabisulfite?

Hi all I'm trying to perform the procedure of sickling-induction in-vitro of peripheral red blood cells from sickle cell Townes mice, and I'm using 2% sodium metabisulfite. Unfortunately the...

20 January 2019 1,214 1 View

Hi, Is it normal when run PROCESS macro in SPSS for moderator (model 1) to get different results from when I run the linear regression in SPSS?

Could you please help me by giving me some advice on this? Thanks.

02 August 2018 2,012 2 View

Hi, can anyone explain to me how I can identify the pixel intensity of each element from a gray SEM image?

I intend to map out the elemental composition of the concrete SEM images. I have information from sem/edx analysis such as the spectrum, weight% and atomic%. From my readings, is it true that I...

12 March 2018 2,028 8 View

Can I use at least 2% of fetal bovine serum in a transfection with Hiperfect?

I'm trying to transfect N1E-115 cells and the HiPerfect protocol advices not to add fetal bovine serum during the transfection phase. Unfortunately, my cells are dying without the FBS. Do you know...

23 October 2017 4,147 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View