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Questions related from Faisal Rashed
I am running EMSAs with different DNA probes (ss/ds/stem-loop etc.). I hybridized and purified the probes, and consistently see my hybridized and PAGE-purified stem-loop probes run faster than my...
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I am treating my cells with a drug that generates about 20-40 uM H2O2. I see a profound activation of gamma-H2AX but no/very low levels of corresponding DNA damage in comet assay. I am trying to...
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Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...
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I am trying to identify potential binding partners of a nitroimidazole compound, which gets entrapped only in hypoxic cells. The drug has a biontin moiety to facilitate pull down of the...
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I am treating my cells with a hypoxia selective drug, which binds to glycolytic and cytoskeletal proteins (actin, tubulin). When I trypsinize my cells after treating them with drug under hypoxia,...
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I am assessing the effects of a hypoxia selective compound in FaDu cells (a HNSCC cell line). When I treated the cells with the compound for 24 hours (at increasing concentrations) under hypoxia,...
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I am trying to assess the effects of a compound as a radiosensitizer, and am interested to see if the compound aids in induction of apoptosis following radiation.
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