Faisal Rashed

3 Questions 6 Answers 0 Followers

Questions related from Faisal Rashed

Can low levels of ROS induce gamma-H2AX without causing DNA damage?

I am treating my cells with a drug that generates about 20-40 uM H2O2. I see a profound activation of gamma-H2AX but no/very low levels of corresponding DNA damage in comet assay. I am trying to...

15 July 2021 6,034 4 View

Proteins not stacking/ entering in a native PAGE. Can anyone help?

Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...

16 January 2021 2,338 4 View

Can senescent cells be stained positive for Calcein AM?

I am assessing the effects of a hypoxia selective compound in FaDu cells (a HNSCC cell line). When I treated the cells with the compound for 24 hours (at increasing concentrations) under hypoxia,...

11 May 2018 3,315 3 View