Do someone know some strong counterions that are not perfluorated for reversed phase chromatography coupled to mass spectrometry ? We already used perfluoroheptanoic acid in a context of analytical set-up to separate polar compounds, but its use lead to interference in other analyses. The column used is HSST3 and has C18 silica beads but specific for polar compounds.
Tara Louis that is a tough one because of the hydrophobic properties of the perfluorinated species. You could try to use the non-fluorinated components and increase the chain length of the ion pair builder (C8, C9) - not a big fan, and you will likely face ion suppression effects in the MS.
So, you are analyzing cationic species, that you would like to retain not using the HFBA. That speaks for small amines or amino components. What we did was use formic acid, but chose a more hydrophobic column. As a matter of fact, we chose a polymeric-based column, as the analytical challenge we faced was not the most complex one, and we could live with the significantly lower chromatographic efficiency. However, we used MS detection as well - I will copy a link to the technical report below, and it worked like a charm.
Hence my suggestion is to go for non-fluorinated chemicals (better for the environment anyway), and choose a more "hydrophobic" column like a C30 modified. In other words, get the chromatographic resolution through the stationary phase, rather than through the ion pair reagent.
I hope this helps!
Good luck with your research!
Detlef.
Technical Report Simultaneous determination of tetrabutylammonium and tributy...
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