Do salt in PBS affect the TEM image quality ?

If I prepare my sample in PBS, do the salt in PBS will affect the TEM image quality?

04 October 2019 9,331 3 View

How to measure Early Voltage of a BJT experimentally?

Hi All, Could you please explain me how to measure the Early Voltage of a BJT experimentally in lab (Undergraduate Electronic Devices Lab)? I am also interested to know the different parameters of...

22 December 2015 5,701 5 View

Doesn't water affect the RAW 264.7 cells?

To induce inflammation in RAW 264.7 cell lines, we treat cells with LPS (lipopolysaccharide) which is supposed to be dissolve in water. Did adding of water to the culture have any effect on cell...

03 February 2015 5,895 4 View

Why are RAW 264.7 cells not growing uniformly in culture flasks?

We cultured RAW 264.7 cells in DMEM medium, but there is no uniform growth in culture flask, all cells are grown as clumps at one place (and many like that through out the plate), there is no...

26 January 2015 8,584 3 View

Can anyone quantify IL-6 or TNFa expression from immunofluorescence microscopic images of cultured cells?

Is image J software the right one or is some other software helpful?

12 April 2014 3,225 6 View

Does anybody suggest to reduce cytosolic staining along with nucleus in the cells stained with vectashield-DAPI reagent for fluorescent micrscopy?

26 December 2013 7,532 1 View

Why is it assumed Z=e^jw and S=jw?

20 December 2013 5,508 21 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View