I want to express a plant secreted protein in yeast and I do not know whether to change or keep the native signal peptide,
thanks!
Hi Pablo S Aguilar
See info below may be useful for you.
Article A yeast-based model system for cloning secreted and membrane proteins
https://bio-protocol.org/e2839
Dear pablo,
I am not sure if the signal sequence of your plant peptide would serve the purpose while expressing in yeast. However, for heterologous expression in yeast, the available vectors would have a signal sequence for efficient expression.
I don't think that peptides carry the signal in yeasts
Dear Pablo S Aguilar ,
I’m afraid that your question relates to a matter that is a bit more complicated than ‘just’ the choice of the signal sequence. See for an excellent review:
Cereghino, J. L., & Cregg, J. M. (2000). Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS microbiology reviews, 24(1), 45-66.
https://academic.oup.com/femsre/article/24/1/45/525926
However coming back to your question. It is clear that several different secretion signal sequences have been used successfully although success rates varied. The S. cerevisiaeα-factor prepro peptide has been used with the most success.
One of the reasons why the native (plant) sequence most likely will not work is because of the differences between signal sequences in different species. We know this thanks to the ground-breaking work of Gunnar von Heijne and his co-workers. See for example:
Emanuelsson, O., Nielsen, H., Brunak, S., & Von Heijne, G. (2000). Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. Journal of molecular biology, 300(4), 1005-1016.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.452.1988&rep=rep1&type=pdf
So I think it is better to choose another signal sequence instead of the native plant sequence.
Best regards.
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