What buffers can I use with the human IGF1 ELISA?

I'm building an ELISA from an antibody pair kit (R&D Duoset DY291) and it seems I can't use BSA in case there's any bovine IGF1 (100% similarity to human form) present so it suggests I use...

19 January 2021 7,867 4 View

Is the first clean up necessary in Illumina MiSeq Library Preparation?

I'm trying to streamline my metagenomics protocols for my lab. I have previously followed the 16S protocol...

16 September 2020 5,234 6 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 9,949 4 View

Does anyone know where to find global temperature projection data in raster format?

Hi all, I'm looking for climate change projection data in raster format. Something like is shown in the IPCC 5th report chapter 12. In particular I'm interested in the Arctic, temperature,...

20 February 2019 8,281 3 View

Why is there coloration in free standing PVDF films prepared by solution casting method?

Can anyone explain why there is coloration of my PVDF films prepared by solution casting method using solvents DMAC & DMSO? I think it may be some sort of degradation of the polymer, I noticed...

29 March 2018 7,377 3 View

Where can I find the key differences for expression between CHO or HEK293 cells following lentiviral transduction?

I will be attempting to stably express a cDNA sequence that codes for a membrane protein following transfection with this...

05 July 2015 5,830 3 View

Do all fluorescent molecules show some level of change with solvent polarity?

Hi,I am currently studying some fluorophores and wanted to check that they don't show solvent dependency. When I change the solvent there is not a change in absorption/fluorescent spectrum by eye...

20 May 2015 2,278 9 View

Is there a specific name for a Probability Mass Function with a single non-zero element?

22 April 2015 9,348 10 View

MEGA 6: Treatment of Gaps/Missing Data - What to choose?

I had difficulty in amplifying DNA stretches due to sub-optimal insect specimens and now have multiple sequences of varying lengths produced from a variety of primer pairs. All do, however, fall...

18 January 2015 4,597 9 View

What is the method to obtain a water retention curve in your lab, country? I am conducting a review. E.g pressure plates, centrifuge etc

Also interested in what people use the data for and what software they use for fitting data.

02 April 2013 2,489 29 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View