What buffers can I use with the human IGF1 ELISA?

I'm building an ELISA from an antibody pair kit (R&D Duoset DY291) and it seems I can't use BSA in case there's any bovine IGF1 (100% similarity to human form) present so it suggests I use...

19 January 2021 7,867 4 View

Is the first clean up necessary in Illumina MiSeq Library Preparation?

I'm trying to streamline my metagenomics protocols for my lab. I have previously followed the 16S protocol...

16 September 2020 5,234 6 View

Anyone interested in Collaborating on Covid-19 Pandemic and Learners' Satisfaction?

The Covid-19 pandemic, described as unprecedented, has forced many educational institutions to switch from traditional or conventional (physical) mode of learning to a more flexible and digital...

15 June 2020 1,258 32 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 9,949 4 View

Does anyone know where to find global temperature projection data in raster format?

Hi all, I'm looking for climate change projection data in raster format. Something like is shown in the IPCC 5th report chapter 12. In particular I'm interested in the Arctic, temperature,...

20 February 2019 8,281 3 View

Why is there coloration in free standing PVDF films prepared by solution casting method?

Can anyone explain why there is coloration of my PVDF films prepared by solution casting method using solvents DMAC & DMSO? I think it may be some sort of degradation of the polymer, I noticed...

29 March 2018 7,377 3 View

What standard is most appropriate for the determination of quantum yeild of FITC dye?

26 April 2017 3,366 4 View

Any recommended alternative to recombinant protein expressions from cloning procedures?

17 October 2016 5,552 2 View

What is the difference between bacteria and animals oxidative phosphorylation?

Animals have mitochondial where oxidative phosphorylation can occur but what happens in bacteria; how do they carry out this process?

25 July 2016 6,846 3 View

Is anyone familiar with cell lines for studying hematopoietic activity?

To detect if an active constituent has hematopoietic potential using cell lines.

31 May 2016 6,292 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

Why is absorbance measured at 540 nm instead of at 280 nm when quantifying proteins in Lowry and Buiret methods?

Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...

16 December 2020 1,010 3 View

Queries regarding Antibody labelling ?

Hello Everyone. I was trying to label antibody with Cat# MO-L007 - Cystein reactive monolith series antibody labelling kit. I followed every steps that the protocol states. But in the final step...

26 September 2020 3,439 1 View

How do I quantify protein extracted from cereal flour with 70% ethanol?

How do I quantify protein extracted from cereal flour with 70% ethanol using Lowry assay ? Standards from protein BSA can not be prepared in ethanol because of low solubility. So which is the...

07 November 2019 4,032 1 View

I have a sample containing mixture of enzymes (powder form) . I want to do protein estimation of the sample. How do I make the stock solution?

How do I make solution from unknown enzymes' mixture for my experiment?

05 June 2019 6,398 3 View

What is the protocol for the extraction of proteins while estimating the total proteins by Lowry's method?

Can anyone share the protocol for extracting the protein from the sample while estimating the total proteins by Lowry's method

04 May 2019 3,139 1 View

I thought I uploaded the following note but I am getting requests for copies. Can you check to see this is publicly available?

full ref below: uploaded a few days ago Gulland, M.D., Thomas, P.O., Stirling, I., DeMaster, D.P., Reeves, R.R., Frost, K. 2019. Lloyd Lowry. Marine Mammal Science 35: DOI: 10.1111/mms.12589

01 February 2019 1,716 1 View

Acc to Lowry's protocol we need to grind the sample in buffer. Can you please suggest me which buffer to select for protein estimation?

Lowry's method

08 September 2018 8,900 1 View

What is the best method for quantifying the total protein concentration in a ligament sample?

I am concerned that using a Lowry assay may not capture all protein content in a ligament sample. Any suggestions for a method? I will be using total protein to standardize a Collagen 3 ELISA.

07 August 2018 8,753 4 View

What is the accurate method for quantification of protein during immobilization?

There is a problem i am getting during the immobilization of protein on support . Initially 1-2mg/mL of protein concentration and 1 mg of support is being taken. After immobilization the protein...

08 July 2018 2,624 3 View