I made protein isolate from my Okra whole four. Did a lowry test on it and I got Protein percent less than 70%. Is this acceptabl?
What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks
18 July 2024 3,581 3 View
Can I publish paper with researchgate?
19 May 2024 697 1 View
In a toxicology study, the EC concentrations are reported as as units of "log μM," for example 0.49 log μM. How do I translate that into simple μM concentrations? In this case, is the base of...
16 July 2023 5,561 2 View
I need services for SEM imaging and Photomicrography of algae
21 April 2023 7,147 0 View
Hello. I was curious what I should do about preprints after they have been peer reviewed and published in a journal... do I delete the preprint, or do you simply change the preprint to an article,...
14 March 2023 8,282 3 View
I am interested in learning of preferred formulations of tissue culture media used to grow human B cells (CD19 cells) isolated from peripheral blood mononuclear cells (PBMCs) of Leukopaks using...
05 March 2023 5,953 0 View
In my lab we’ve been working at trying to label a protein with Cy5-maleimide for a long time. The protein has a single native cysteine residue that doesn‘t undergo the labeling reaction with any...
02 February 2023 6,341 0 View
I need a detailed breakdown of how I can get the CTE of a sintered sample from the obtainable data after the use of SPS machine to sinter. I am finding it difficult to manipulate the relative...
04 July 2022 5,168 2 View
Currently working and I'm not open to a freeze dryer to dry my protein isolate. Can I airdry. Will the protein content not be affected?
10 February 2022 901 3 View
Due to increase in vaginal infection
07 July 2021 3,649 3 View
What means gamma in old chemistry papers like Lowry method? I have a suspicion it was the concentration unit in micro something. I am right? Why they used it? Typographical problems at the time?
22 January 2024 9,232 2 View
We need to accurately measure the protein/peptide concentration in a sample that is at least 80% DNA (primarily ssDNA less than 80 bases long with some dsDNA also). A majority of the methods I've...
24 September 2023 9,156 3 View
please share an easy-to-follow protocol of lowry assay, along with the method to prepare BSA standard graph in the range of 5ug/ ml to 100ug/ml
16 August 2023 8,572 0 View
I am trying to check a purified recombinant protein on SDS PAGE coomasie Blue and silver staining. Before SDS PAGE, I have checked the total protein concentration using lowry and it shows good...
03 July 2023 8,987 4 View
Iam working on seaweed protein. After extraction of protein, i have precipitated with ammonium sulphate and freeze dried the sample after dialysis. In kjeldahl iam getting good percentage of...
18 April 2023 7,610 0 View
Hi all, I am looking into purchasing the Thermo Scientific Pierce Modified Lowry Protein Assay Kit (Catalog number: 23240) to quantify total protein amount in mammalian cell lysates. I lyse my...
14 April 2023 7,998 7 View
Hello to everyone. My work focus on studying PTMs and I have successfully phosphorylate my protein in vitro (Checking the reaction products by SDS-Page, all of my protein should be...
29 March 2023 1,640 3 View
Special greeting. I have a protein extraction solution containing 1% SDS, 300 mM B-mercaptoethanol, 100 mM Tris and 8 M urea, 10 mM EGTA. Is there a method that allows me to measure protein...
28 March 2023 2,338 4 View
1. Basic concept of Crude protein and soluble protein? 2. Is lowry et al 1951 method used for estimation of Total soluble protein content in mungbean?? 3. If method has been changed then value...
04 March 2023 4,258 0 View
I used Lowry Technic. I prepared standards from BSA for make up standard curve. And then, ı measured my samples proteins. How can ı find protein contents in my samples?
17 January 2023 2,199 2 View