What buffers can I use with the human IGF1 ELISA?

I'm building an ELISA from an antibody pair kit (R&D Duoset DY291) and it seems I can't use BSA in case there's any bovine IGF1 (100% similarity to human form) present so it suggests I use...

19 January 2021 7,867 4 View

Is the first clean up necessary in Illumina MiSeq Library Preparation?

I'm trying to streamline my metagenomics protocols for my lab. I have previously followed the 16S protocol...

16 September 2020 5,234 6 View

Anyone interested in Collaborating on Covid-19 Pandemic and Learners' Satisfaction?

The Covid-19 pandemic, described as unprecedented, has forced many educational institutions to switch from traditional or conventional (physical) mode of learning to a more flexible and digital...

15 June 2020 1,258 32 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 9,949 4 View

Does anyone know where to find global temperature projection data in raster format?

Hi all, I'm looking for climate change projection data in raster format. Something like is shown in the IPCC 5th report chapter 12. In particular I'm interested in the Arctic, temperature,...

20 February 2019 8,281 3 View

Why is there coloration in free standing PVDF films prepared by solution casting method?

Can anyone explain why there is coloration of my PVDF films prepared by solution casting method using solvents DMAC & DMSO? I think it may be some sort of degradation of the polymer, I noticed...

29 March 2018 7,377 3 View

What standard is most appropriate for the determination of quantum yeild of FITC dye?

26 April 2017 3,366 4 View

Any recommended alternative to recombinant protein expressions from cloning procedures?

17 October 2016 5,552 2 View

What is the difference between bacteria and animals oxidative phosphorylation?

Animals have mitochondial where oxidative phosphorylation can occur but what happens in bacteria; how do they carry out this process?

25 July 2016 6,846 3 View

Is anyone familiar with cell lines for studying hematopoietic activity?

To detect if an active constituent has hematopoietic potential using cell lines.

31 May 2016 6,292 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

Can i use the " weight moisture" ( humidite ponderale in French) of plants to describe their efficiency in using water?

Weight moisture is : water content/ dry weight , if for example it is 4 it means that this plant have for each 1gam of dry mass 4 grams of water. can i use this to interpret the plant use of...

14 February 2021 5,784 1 View

Why (111) peak of ZeoliteX changes depending on dry or wet?

09 February 2021 6,307 3 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View