Do our isolated marine sponges associated with Streptomyces sp. 16s rRNA sequence fall under uncultured or cultured prokaryotic sources?

More Nagendra sastry Yarla's questions See All

Autodraw in OSLO software?

Hello everyone, I would like to ask you a very simple question about OSLO software for optical designing. Here I have attached an Autodraw window for an objective lens. Can anyone explain in the...

20 September 2023 6,348 2 View

What strategies are lacking for implementation of sustainable cooling solutions in cities and regions?

20 May 2023 2,620 2 View

Reseach Square is any legal agency of publication? Have any index and Impact Factor?

Why Research Square forces to researcher join the team? Its any useful? some papers online without acceptance in Research Square

05 May 2023 3,730 4 View

Can we calculate energy of any particular molecule?

If we take a protein or peptide, can we calculate energy without excited in higher stage?

31 January 2023 8,450 2 View

How to test laser intensity profile image is a Gaussian distribution ?

I am from Physics, background and doing some experiment using a laser source. Using a collimating lens, I have made the beam as a collimated beam. The intensity distribution of collimated beam is...

11 January 2023 7,347 11 View

How can we extract water soluble compounds during extraction highly polar compound like amino acid?

How can we extract water soluble compounds during liquid extraction but pH of Prduct highly basic up to 13 and product Highly soluble in water?

13 June 2021 5,736 3 View

Many people recovering from COVID-19 have of late been afflicted by black fungus or mucormycosis disease. What can be the exact reason?

Recently in India many people recovering from COVID-19 have of late been afflicted by black fungus or mucormycosis disease. What can be reason? how to diagnosis in early stage?

17 May 2021 6,958 26 View

What is main difference between COVID-19 effected patient fever body temperature and typhoid fever body temperature?

We are writing report on COVID-19 fist, second and third wave in different country of the world.

21 April 2021 4,665 2 View

How to extract the on current and off current of TFET form the output results Vds vs Ids and Vgs vs Ids in silvaco Atlas?

i am trying to know the On current and off current of TFET form its characteristics in silvaco Atlas?

19 April 2020 4,453 1 View

How to add a user defined material with high dielectric value in silvaco atlas ?

procedure to add different high dielectric constant material in the silvaco atlas?

16 January 2020 3,629 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View