Does anyone have the experience of isolating some human PBMC subsets (CD4+ T cells, NK, Macrophage) by positive selection using microbeads and then using them for in vivo experiments (injecting into NSG mouse)? Do microbeads affect their function? Thank you.
Can you do a negative selection instead? That would eliminated any potential affect to function. Anecdotally, I've seen changes in NK cell activation status after positive selection performing similar experiments.
Negative selection can not achieve the purity I need, because even very few non-specific cells might expand in mouse.
For functional assays it is always better to use negative selection. There are magnetic kits which results in very good purity. I have this experience with NK cells.
- Badges
- Science topic