Do inactive proteins often become thermostable?

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How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

What are the roles of both Monetry and Fiscal Policy coordination in optimising the Macroeconomic outcomes in the economy ?

These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...

30 July 2024 2,104 6 View

Is CFI / TLI = 1 okay in Mplus?

Hi there, I did a CFA to 10 items on 2 latent variables. The model fit is too good and I've never seen it before. CFI / TLI = 1, RMSEA = 0. Is it okay, if everything else was done properly?...

24 July 2024 5,454 7 View

How to make soil water characteristic curve using centrifuge?

We have no access of pressure plate apparatus

22 July 2024 3,488 2 View

What is the degassing temperature and time for biochar for BET surface area analysis if TGA is not possible?

Since BET and TGA both are very costly instruments and many places the facilities are not available (for large number of samples) hence a threshold degassing temperature and time is preferable

16 July 2024 8,112 2 View

How to prepare alcohol foam with high alcohol content?

Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...

15 July 2024 3,539 0 View

Researchers, have you come across any intriguing but underexplored research topics that you would like to share?

Your ideas could spark the next big thesis or dissertation for students eager to make an impact in their field.

14 July 2024 9,999 0 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

Suggestions for storage of CD4+ naive splenocytes for future use?

Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...

02 July 2024 4,887 0 View

How does the melting point of a spherulite correlate with its size?

For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...

30 June 2024 2,891 1 View

How to reduce the material coming out of the torque rheometer?

A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....

25 June 2024 10,154 0 View

Why is my TE-eluted cloned plasmid (isolated via miniprep) not freezing at -20 degree celsius? Is it normal?

I isolated my cloned plasmid from TOP10 cells using invitrogen plasmid miniprep kit and eluted it in TE buffer. But I noticed that contrary to my plasmid aliquot, it does not freeze at -20 degree...

19 June 2024 1,612 8 View