2 Questions 3 Answers 0 Followers
Questions related from Swati Gupta
I tried a pH of Tris 8.8 pH but after TEV cleavage, I find there is less cleavage and not much desired protein. Which buffer should be used so that no changes in between would be done to prevent...
07 April 2018 2,958 5 View
Am getting a chaperone at around 63 kD . I have my construct in pETM41. Am purifying with amylose resin and getting a chaperone after affinity. Will HIC after first step clean my protein?
22 July 2017 899 4 View