Swati Gupta

6 Questions 5 Answers 0 Followers

Questions related from Swati Gupta

Do inactive proteins often become thermostable?

I had made a mutant that was inactive but thermostable with an increased melting temperature of ten degrees from the wild type. Could the inactivity lead to a structural change in conformation...

01 April 2021 4,655 3 View

Hello, I am working with a protein which has an MBP tag with pI 7.5; without tag the pI of only protein is 8.4. Which buffer shud use Tris 7?

I tried a pH of Tris 8.8 pH but after TEV cleavage, I find there is less cleavage and not much desired protein. Which buffer should be used so that no changes in between would be done to prevent...

07 April 2018 2,978 5 View

Hello < I am trying to standardize IFA with Plasmodium falciparum parasitized RBCs . Can you help in that?

And am using 1:400 dilution for my protein's antibody. With overnight 4 deg binding. Should I make it 1 hour at RT. I increased tween in PBS from 0.01 to 0.05 % . I got some less background. But...

07 October 2017 3,870 0 View

How much micromolar tRNA should I use for crystallisation with a protein?

I have a tRNA binding protein which i need to set up tryas with commercial tRNA. Please let me know some concentrations

28 July 2017 6,382 2 View

Does HIC help to get rid of chaperones?

Am getting a chaperone at around 63 kD . I have my construct in pETM41. Am purifying with amylose resin and getting a chaperone after affinity. Will HIC after first step clean my protein?

22 July 2017 934 4 View

Can I purify an N terminal His-MBP fusion construct in pETM41 with Ni-NTA instead of amylose resin?

I needed to know if that affects the yield of the protein

18 July 2017 8,429 2 View