Swati Gupta

2 Questions 3 Answers 0 Followers

Questions related from Swati Gupta

Hello, I am working with a protein which has an MBP tag with pI 7.5; without tag the pI of only protein is 8.4. Which buffer shud use Tris 7?

I tried a pH of Tris 8.8 pH but after TEV cleavage, I find there is less cleavage and not much desired protein. Which buffer should be used so that no changes in between would be done to prevent...

07 April 2018 2,957 5 View

Does HIC help to get rid of chaperones?

Am getting a chaperone at around 63 kD . I have my construct in pETM41. Am purifying with amylose resin and getting a chaperone after affinity. Will HIC after first step clean my protein?

22 July 2017 898 4 View