Hi all,

This seems to be a fundamental thing but I have this doubt. I have biological replicates of a timecourse experiment with a particular treatment. I wanted to know if I can combine both to make a protein lysate and then run a Western or treat them separately? We usually use both the biological replicates while doing a quantitative assay like Real time PCR or ELISA. But since WB is a semi-quantitative method, is it really necessary to have/run both the biological replicates?