Anand Vivek Desai

10 Questions 17 Answers 0 Followers

Questions related from Anand Vivek Desai

Do I need to use both biological replicates for Western Blot?

Hi all, This seems to be a fundamental thing but I have this doubt. I have biological replicates of a timecourse experiment with a particular treatment. I wanted to know if I can combine both to...

01 January 2017 4,553 3 View

Why don't I see RNA on my gel?

I have a RNA sample with 300 ng/uL of concentration and 260/280 as well as 260/230 ratios higher than 1.9. I ran an agarose gel to test the quality of my RNA sample and my loading buffer had a...

01 January 2017 7,699 15 View

Differential amplification with identical PCR conditions?

Hi all, I did this PCR with my reference and test DNA. I had tested the two primer pairs with my reference DNA and they worked pretty well. I ran the PCR and obtained bands with different band...

01 January 2017 4,509 3 View

How to troubleshoot no amplification?

Hi. I ran four PCR reactions with same template and four different primer pairs. Of those, one pair had optimal annealing temperature at 56.5 degrees, while rest of the three had 58, 58.4 and 58.1...

01 January 2017 6,642 9 View

Is BCA assay compatible with lipids in sample?

Hi all, I have cell lysates from adipocytes and I wish to run Western Blot for them. My question is, which is the best method to quantify protein by avoiding interference from lipids?

01 January 2017 1,256 5 View

SDS-PAGE gel stopped running, will my protein band be ok?

I was running a 10% SDS-PAGE gel overnight. It stopped after about five hours of run in the midnight and I realized it when I returned to the lab about 7-8 hours after the gel might have stopped...

12 December 2016 5,637 13 View

Can I use overnight grown culture kept at 4 degrees to prepare glycerol stock?

I have three tubes of 5 mL each of starter culture of overnight grown bacteria with three different plasmids stored at 4 degrees. I was curious if these cultures can be used to prepare glycerol...

10 October 2016 9,376 3 View

How exactly do the agonistic or antagonistic antibodies work?

What is the key difference between these two types of antibodies, if both are immunoglobulin G molecules? What makes binding of one antibody inhibit any given receptor and binding of another one...

04 April 2016 9,503 4 View

Can we use paraformaldehyde as a fixing agent instead of methanol in gel staining solution after SDS-PAGE?

Since the function of both paraformaldehyde and methanol is "fixation", can we replace methanol with paraformaldehyde in the traditional gel staining solution used after SDS-PAGE?

04 April 2016 9,952 3 View

Is there any enzyme/enzymes in Human RBC's that use NADH as a substrate?

I am currently in search for any RBC enzyme other than NADH-Cytochrome b5 reductase, that might use NADH as a substrate or use reducing equivalents from NADH for subsequent reaction. Please let me...

04 April 2016 1,895 5 View