Do I need to remove the domain in my sequence in order for crystallization purpose?

29 February 2016 1 7K Report

Hi guys, I'm trying to crystal out one of the DNA polymerase protein from Bacillus sp. I found out that my DNA polymerase sequence actually contained 5'-3' exonuclease, N-terminal resolvase-like domain and 5'-3' exonuclease, C-terminal SAM fold using Pfam software. Do I need to remove them just for crystallization purpose? Will them affect the crystallization process?

I had attached the result I got from Pfam at below. TQ!

Wafa Ali Eltayb

hi there

i guess would be better to crystallize as full length, coz some domain are crucial for protein folding and package

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