Alex Peh

10 Questions 16 Answers 0 Followers

Questions related from Alex Peh

Is dead cell in flow cytometry affect subsequent immune cell population count?

Hi all, I have a naive question and would like some opinions. I performed 2 flow cytometry runs with different samples, and I found more dead cells in my second run. My question is will the...

06 November 2023 3,148 2 View

Crystal violet staining?

Hi expert out there! I'm using crystal violet staining to determine gram +ve and -ve bacteria, which is a very traditional method, but at the same time, I noticed that crystal violet also can use...

06 May 2021 4,275 11 View

Generation of ApoE-GFP transgenic Line?

Does anyone here can provide me some background for generating the transgenic line in zebrafish? I wish to generating GFP specific expression in microglia by inserting EGFP marker in...

11 October 2017 8,760 1 View

How to get Ramachandran plot statistic?

Hi all, do anyone know how can I get the Ramachandran plot statistics below the interactive plot? I used the SAVES- Ramachandran Plot software (http://services.mbi.ucla.edu/SAVES/Ramachandran/)...

07 March 2016 2,685 2 View

Do I need to remove the domain in my sequence in order for crystallization purpose?

Hi guys, I'm trying to crystal out one of the DNA polymerase protein from Bacillus sp. I found out that my DNA polymerase sequence actually contained 5'-3' exonuclease, N-terminal resolvase-like...

29 February 2016 6,824 1 View

Do anyone know what is this circled in red box?

I'm doing the homology modelling using YASARA for my protein (it's a DNA polymerase from geobacillus). The model show the front tail after I adding the amino acid myself which was belong to my...

24 February 2016 3,744 6 View

What is the protocol using ammonium sulfate for purification?

Had anyone here tried out using ammonium sulfate to purify your protein? How are you actually work on it? Carry out using affinity purification first then salting out the remaining impurities?...

25 November 2015 2,268 6 View

How do I purify my protein?

Hi guys, I tried using 30mM imidazole of binding buffer to perform affinity purification. After the purification, I found out that the band in red box(impurities) from eluent getting darker as...

24 November 2015 9,148 17 View

Will DNA polymerase denature?

Hi guys, I'm going to qualitatively test my DNA polymerase with PCR. But something cross my mind, will my DNA polymerase denature since it is a protein? And I assume it should be quite heat...

02 November 2015 3,392 4 View

Why we need binding buffer and lysis buffer during purification?

Hi all, I was wondering why there is a need for lysis buffer to break the cell and binding buffer for the column affinity chromatography purification? I'm using sonification to break the cell. Do...

11 October 2015 8,455 11 View