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Questions related from Alex Peh
Hi all, I have a naive question and would like some opinions. I performed 2 flow cytometry runs with different samples, and I found more dead cells in my second run. My question is will the...
06 November 2023 3,099 2 View
Hi expert out there! I'm using crystal violet staining to determine gram +ve and -ve bacteria, which is a very traditional method, but at the same time, I noticed that crystal violet also can use...
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Hi all, do anyone know how can I get the Ramachandran plot statistics below the interactive plot? I used the SAVES- Ramachandran Plot software (http://services.mbi.ucla.edu/SAVES/Ramachandran/)...
07 March 2016 2,648 2 View
Hi guys, I'm trying to crystal out one of the DNA polymerase protein from Bacillus sp. I found out that my DNA polymerase sequence actually contained 5'-3' exonuclease, N-terminal resolvase-like...
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I'm doing the homology modelling using YASARA for my protein (it's a DNA polymerase from geobacillus). The model show the front tail after I adding the amino acid myself which was belong to my...
24 February 2016 3,695 6 View
Had anyone here tried out using ammonium sulfate to purify your protein? How are you actually work on it? Carry out using affinity purification first then salting out the remaining impurities?...
25 November 2015 2,224 6 View
Hi guys, I'm going to qualitatively test my DNA polymerase with PCR. But something cross my mind, will my DNA polymerase denature since it is a protein? And I assume it should be quite heat...
02 November 2015 3,353 4 View
Hi all, I was wondering why there is a need for lysis buffer to break the cell and binding buffer for the column affinity chromatography purification? I'm using sonification to break the cell. Do...
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