Do I need to add osteogenic induction media to hFOB1.19 (CRL-11372) cell line to hFOB1.19 (CRL-11372) cell line if I want the cells to differentiate to mature osteoblast? or these cells are osteoblast already? This is my first time hOB cell line culture. Some studies incubate at temperature 39.5°C for differentiating temperature. Is it necessary? or is it OK with Growth Conditions at temp of 34°C? ( recommended by manufacturer )
- If osteogenic media need to be added the media would contains Ham's F12 Medium Dulbecco's Modified Eagle's Medium, with 2.5 mM L-glutamine (without phenol red), 0.3 mg/ml G418, 10% fetal bovine serum supplemented with 0.1 µM dexamethasone, 50 μM ascorbic acid and 10 mM β-glycerol phosphate
Thank you
Hello.
The hFOB1.19 (CRL-11372) cell line is a human osteoblastic cell line. These cells are derived from the bone marrow stroma of a patient with osteogenic sarcoma and can be induced to differentiate into mature osteoblasts when grown in the presence of osteogenic induction media.
So, Yes, you need to add osteogenic induction media to hFOB1.19 (CRL-11372) cell line in order to induce these cells to differentiate into mature osteoblasts. This can be done by adding a combination of compounds that are known to promote osteogenic differentiation, such as ascorbic acid, beta-glycerophosphate, and dexamethasone.
The medium that you provided contains the required osteogenic induction factors: ascorbic acid, beta-glycerophosphate, and dexamethasone, and in the recommended concentrations (50 μM ascorbic acid, 10 mM β-glycerol phosphate, and 0.1 µM dexamethasone respectively).
It is important to note that you also need to keep an eye on the timing of the induction and the change of medium. Osteogenic induction should be done in early passage, high-density cultures, and the induction medium should be changed every 2 to 3 days to maintain the optimal concentration of osteogenic factors. Also, make sure to check the osteogenic markers of the cells after 2-3 weeks, such as ALP, osteocalcin and collagen type I, to confirm the osteogenic differentiation.
It's also important to note that there are some variations in the osteogenic induction media and protocols, and it's always good to check for published articles, protocols, or contact the suppliers/ manufacturers for recommendations.
In summary, hFOB1.19 (CRL-11372) cell line can differentiate into mature osteoblasts when grown in the presence of osteogenic induction media. and the medium you provided contains the factors known to promote osteogenic differentiation: ascorbic acid, beta-glycerophosphate, and dexamethasone. It is important to consider the timing of induction, the change of medium, and monitoring the osteogenic markers to confirm the osteogenic differentiation.
Regards
https://www.researchgate.net/profile/Mohammad-Alzeyadi-2
lse du Preez1,2*, Wim Richter1, Dirk van Papendorp2, Annie hFOB 1.19 osteoblast cells grown on a biomimetic biphasic nanoscaffold: anin vitro evaluation for possible bone tissue engineeringBiomedical Research 2018; 29 (11): 2442-2448
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