What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

What is the experience of clinically using Silver Diamine Fluoride (SDF) to stop caries progression in children's teeth?

Kids globally suffer from dental caries and pose problems for the dentist to treat it with conventional drilling. SDF is recommended to stop progression of carious lesion with out drilling. It...

04 December 2019 4,372 4 View

How can I measure the wavelength of Formaldehyde?

How can I measure the wavelength of formaldehyde in Aqueous solutions by UV- Vis spectrophotometer, and how much amount of distilled water and formaldehyde can use for this measuring

20 July 2019 983 3 View

Is Octa publishing company a reputable publishing company?

I have send them my manuscript for editing and publishing but now I have some doubts about there work

13 May 2019 8,873 4 View

What is the best data center to download regional groundwater level data in the Nile Basin area?

I search for a data center that provides regional groundwater level data for the Nile Basin area especially upper Blue Nile Basin area. I am a bit confused as many published papers do not provide...

26 March 2019 2,841 1 View

What is the best and efficient way to clean graphene surface after growing biofilms on it?

I am using graphene sensor to identify biofilm strains. Need to know what are best methods to cleanse the graphene surface to get device ready for next run.

26 May 2018 5,764 3 View

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28 March 2017 7,717 3 View

What is the relation between pressure difference made by the fan and fan speed (or tip speed)?

Hello I'm trying to make a simulink model for Venturi tube, for the purpose of measuring volumetric airflow by making a pressure difference with a fan. The paper "Model-based analysis and...

13 December 2016 9,768 6 View

Does difference between GC content of primer and PCR product affect PCR efficiency?

Does difference between GC content of primer and PCR product affect PCR efficiency?

17 June 2016 9,971 5 View

Can high energetic particles be made to reflect of a laser sheet of light?

I know of laser light reflecting off or being scattered by particles, but is it possible that particles reflect of a sheet of laser light? For example in the experiments of ultracold atoms in a...

14 January 2016 3,925 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View