Why HRLDAS need to combine snow and rain as Precipitation? Why this need hourly interpolation of solar radiation?

Dear coworkers, my questions are about HRLDAS. I am runing HRLDAS with GLDAS-based forcing data. For running offline LSM such HRLDAS, we know we need atmospheric forcing and initial data. In...

28 March 2020 8,726 3 View

Can anyone recommend a good caspase-3 antibody?

I'm looking for an antibody that can detect both procaspase-3 (>30kDa) and active caspase-3(>15kDa) using western blot analysis. Did you test this experimentally? Did you get good results using a...

20 January 2020 112 3 View

How to treat poly(I:C) (polyIC, poly-IC, polyI:C) to NS-SV-AC or other cell line?

Hi, I'm experimenting to check the effect of treating polyIC (poly(I:C), polyIC, poly-IC, polyI:C) to NS-SV-AC cell lines. But I haven't been witnessing a significant cell death after treating...

23 July 2019 1,690 1 View

Is impacting the only option for experimental modal analysis of structure with free-free boundary condition?

For the modal testing of free boundary condition structure, such as a steel beam put on the bungee cords, is it possible to apply force using the actuator, like modal shaker? I guess it is...

24 June 2019 3,806 3 View

How can I estimate the purity of a recombinant protein after purification process using size-exclusion chromatography (no standard product exists)?

I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...

23 April 2019 4,383 12 View

How to decide sample size for validation?

I have data having over 20000 rows. 1) I am going to use a tool to test how many error rates this tool will calculate in this data. 2) And then, I will randomly choose some data and check how...

24 March 2019 7,656 4 View

How can i use EMBL pride inspector?

Hellow everyone. I am supposed to use EMBL pride proteomics documents. But I don't know how to use EMBL pride inspector program. even there is no file to introduce inspector program. Up to now, I...

29 May 2018 4,754 2 View

Are there any tries or results about carbon nanotubes film prepared through vacuum thermal evaporation?

I am searching some tries related to it. But most of the search results are about a synthesis of the carbon materials itself via chemical vapor deposition (CVD).The term 'CVD' contains 'vapor' so...

19 June 2017 3,463 2 View

Looking for a polymer with specific properties

Could anyone suggest a polymer having following properties? 1. It should be easily soluble in water at 20 degree C 2. It should NOT be soluble in ethanol 3. It's melting point must be well above...

11 June 2017 3,547 5 View

Why flame synthesized soot is more stably adhere at quartz glass than amorphous glass?

Recently, I tried to gather soot (carbon black) at slide glass through alcohol lamp burning. The glass was positioned over flame of wick (about 5 cm from the wick). Under amorphous glass...

06 April 2017 1,837 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

How to formulate Energy minimization in Molecular dynamics simulation for alloys using meam potential?

during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...

22 February 2021 380 5 View