Do High or low A260/A230 and 260/280 ratio for isolated RNA make problems in real time PCR results?

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Can Kiessling

Check out previous discussion on this topic:

https://www.researchgate.net/post/Can_low_260_230_ratio_in_RNA_extraction_interfere_with_qPCR_or_RT-PCR_result

https://www.researchgate.net/post/Does-low-260-230-affects-ct-value-in-real-time-PCR

https://www.qiagen.com/us/resources/faq?id=c59936fb-4f1e-4191-9c16-ff083cb24574&lang=en

https://www.qiagen.com/us/resources/download.aspx?id=11226191-0a82-4a9b-ba4a-99800b6f8595&lang=en

Parisa Kadkhodaei Elyaderani

Can Kiessling Tnx dear for your guide

Mohit Gupta

Hi Parisa,

As per my understanding low or high RNA purity and concentration ratios can interfere with reverse transcription and PCR amplification, leading to inaccurate results. Clean up your RNA before performing real-time PCR.

Best of luck!!