Do epithelial cells secrete proteins that degrade elastin?

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Steingrimur Stefansson

Hi Ariane, epithelial cells secrete metalloproteinases that can degrade elastin.

To identify them, you can try doing a elastin zymogram SDS-PAGE as described in these papers:

http://www.jbc.org/content/266/12/7870.full.pdf

http://atvb.ahajournals.org/content/21/8/1269.full

You can also try a quenched elastin substrate for measuring elastase activity in solution:

https://tools.lifetechnologies.com/content/sfs/manuals/mp12056.pdf

Avinash Sheshachalam

http://www.ncbi.nlm.nih.gov/pubmed/15474460

MMP12 is secreted by bronchial epithelial cells

Ariane Neumann

Dear Steingrimur, dear Avinash, thanks for the very helpful answers!!!

Best regards!