Do different proteases have different cleavage site in an antibody/fusion protein? Literature would be helpful.
Many thanks,
Harleen
Emil G P Stender
Hi Harleem
Some proteases are more specific than others. If you have the primary structure of your protein I suggest trying this tool:
https://web.expasy.org/peptide_cutter/
Please note that it assumes complete degradation, which properly folded proteins will be resistant to. Hope that was helpful
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Alireza Mordadi
Absolutely yes, we have six classes of protease.
Take a look.
https://www.researchgate.net/publication/206760853_Protease_-_Revisting_the_Types_and_Potential
Regards.
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Shamsher S. Kanwar
Yes, it's very likely that different proteases have different site of action on target protein. It's true with AB and fusion protein too.
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