How to determine whether to use isocratic or step gradient method for HPLC based methods?

How to determine whether to use isocratic or step gradient method for HPLC based methods?

08 September 2019 8,457 4 View

Do different proteases have different cleavage site in an antibody/fusion protein?

Do different proteases have different cleavage site in an antibody/fusion protein? Literature would be helpful. Many thanks, Harleen

06 July 2019 1,973 3 View

What is the pressure displayed on HPLC panel and empower - Pressure required to push the sample or Back Pressure?

What is the pressure displayed on HPLC panel and empower during sample flow - Pressure required to push the sample or Back Pressure generated inside the column?

06 July 2019 3,559 13 View

Absence of protein band in zymogram?

Hi, I'm running a 10% zymogram gel. On running the harvest sample and flow through sample (prior to protein purification), I observe the metalloproteinases band at 85kDa and 50kDa....

06 July 2019 7,291 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View