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Questions related from Harleen Kaur
How to determine whether to use isocratic or step gradient method for HPLC based methods?
09 September 2019 8,589 4 View
Hi, Does anyone uses Welch column 2.0 * 30mm, 30um for protein A analysis? If so, what is the column chemistry? Regards, Harleen
08 August 2019 3,878 3 View
Hi, I'm running a 10% zymogram gel. On running the harvest sample and flow through sample (prior to protein purification), I observe the metalloproteinases band at 85kDa and 50kDa....
07 July 2019 7,350 10 View
Do different proteases have different cleavage site in an antibody/fusion protein? Literature would be helpful. Many thanks, Harleen
07 July 2019 2,033 3 View
What is the pressure displayed on HPLC panel and empower during sample flow - Pressure required to push the sample or Back Pressure generated inside the column?
07 July 2019 3,618 13 View
