I am working on adherent breast cancer cells. Just want to know if the attachment will take place without a protein
I'm confused by what you are trying to do? Why would you try to attach cells to polyacrylamide gel (PAG)? The boring answer to your question is it depends on the cell line. I suspect most cells would not adhere to PAG without proper ECM coating. To assess adhesion, plate cells on PAG for x amount of time. The was extensively and count cells attached (you can stain with the attached cell with crystal violet for example). For viablity, you can stain cells with trypan blue.
Hi,Ask yourself two different questions: How many publications do you know where cells have been cultured on polyacrylamide without ecm proteins? And: How many scientists do you know who add an additional step in a protocol because they like the extra work? ;-)To answer your question: Cells do adhere very poorly to polyacrylamide. If you would be lucky and left them long enough, some might get around to produce their own ecm and survive, but I think you would probably save yourself some time sticking with established protocols.@Richard: its an assay often used at different bisAcrylamide / Acrylamide ratios to look into the cellular responses to mechanically different environments.
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