Vandhana Ashok

5 Questions 2 Answers 0 Followers

Questions related from Vandhana Ashok

How do I maintain single cell isolated colonies in good health for long period of time?

I am trying to isolate single cell colonies from breast cancer cell lines. After isolating , I let them grow in 96 well plates. Initially for a week they are fine. But as days pass, the colonies...

02 February 2016 8,698 4 View

Why is my gelatin zymography gel half stained and half not stained with coomassie?

Is there any specific way to dissolve gelatin? can someone give the procedure to prepare gelatin?

12 December 2015 4,751 2 View

Do cells attach to polyacrylamide gel without an ECM coating? How do I assess these cells on terms of viability & adhesion?

I am working on adherent breast cancer cells. Just want to know if the attachment will take place without a protein

05 May 2015 6,822 2 View

Can cancer stem cells and side population cells be differentiated?

Are they the same and could cancer cells be both these kind of populations?

07 July 2014 4,805 3 View

What are the techniques used to identify single cell clones from 96 well plate after limiting dilution?

I want to identify clones from a cell line. I need to know the techniques to identify if the cells I separate are the same or different clones.

06 June 2014 9,812 3 View