Hello All,
I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell line could be used?
Hello, Sakthi!
human NK cell line KHYG-1
Article A Novel Assay for Antibody-Dependent Cell-Mediated Cytotoxic...
file://b40.med.local/profiles/RDS_folders/chernovan/Downloads/s41598-018-36972-2%20(1).pdf
Article NK-mediated antibody-dependent cell-mediated cytotoxicity in...
Thank you so much Alexandr Chernov. Really appreciate your help!
I am intertesting
In the area of material science and manufacturing engineering.
08 July 2024 5,576 0 View
I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...
05 June 2024 691 1 View
If u see nature ex:- honeycomb it is made of hexagon which on heating forms a sphere but y not in crystallography?
26 May 2024 6,604 5 View
ER (Entity Relationship) Diagram helps to find the path path between one entity to another entity, or one attribute to another attribute. But most of the ER Diagram has only one direction to...
26 May 2024 8,663 0 View
Reason for the disappearance of O-H peak in NMR
24 April 2024 5,816 5 View
In fluorescent chemosensor, why the designing of the prob is getting difficult in different solvent environments?
24 April 2024 2,812 1 View
It is from 7-hydroxy-4-methyl coumarin with HMTA in dil acetic acid at 90 degrees for 6 hrs and hydrolysed with 20% HCl, kept for 30 min. cooled overnight and extracted with ether.
15 April 2024 3,941 0 View
Most of the AI2SQl automatically generates SQL Query based on the training data. I want to generate a Query with the consideration of the Values present inside the table. Suppose a value called...
08 April 2024 5,731 3 View
I currently have the correlation/covariance matrix for a set of variables, as well as the output from a regression analysis, but lack access to the underlying raw dataset. Given these...
03 March 2024 6,116 4 View
In microbial fuel cell Which type of electrodes and membranes can be used and what is the material (waste) is taken.
12 February 2024 5,956 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View