My question in the concern to evaluate the cellular transformation in the cell lines in a tissue culture lab?
Hi, it is depending on the origin of cells and the method of cell separation .Actually most of the cell lines are a mixture of different types of cells. I think monoclonal cell lines are consisting from one type of cells. Also you can separate your cells by cell sorting or magnetic beads to produce a population of homogeneous cells. In most of the time, if you use cells of a tissue directly, you will have a mixture of different types of cells.
If you mean cell culture medium, answer is NO. All cells are in same cell line are same. But cells are different in different cell lines.
Hi, it is depending on the origin of cells and the method of cell separation .Actually most of the cell lines are a mixture of different types of cells. I think monoclonal cell lines are consisting from one type of cells. Also you can separate your cells by cell sorting or magnetic beads to produce a population of homogeneous cells. In most of the time, if you use cells of a tissue directly, you will have a mixture of different types of cells.
Well all the cells in a cell line SHOULD be the same for all practical purposes and experiments. However you are right in assuming that there are transformations possible due to prolonged culture. If you are dealing with a new culture and do not know its status, it is advisable to check the ploidy of the cells after ever 15 passages. And this can be done using a simple flow cytometric analysis using the PI stain.
Hope this helps. All the best.
i suggest check the karyotyping result of a cell line in ATCC ie. they always mixed at genetic level. this is true mostly for cancerous cell lines.
@ Md. Nurunnabi.... If you are talking in theoretical manner...its fine on the papers and books.....But practically scenario is really different as there are cellular transformations.
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