I want to know, what are the binding coefficients in mouse for the calculation of free 25(OH)D? Please help me
Hi Anirutha. serum albumin is the main serum transport protein for ALL hydrophobic metabolites. Their Kd is around 10-3 t0 10-4 M because they all bind to similar sites in serum albumin.
Hi One of my friend had kept 2 ml of filtered incomplete DMEM media for check for 6 days in a 35 mm UV sterilized petri plate (Kept in UV for 1 hour). He had also checked before keeping it in the...
27 February 2020 698 0 View
I am working on a drug called Temozolomide in my cell culture experiments. I am having trouble in preparing working concentrations of this drug from 200 to 2000 uM as the drug is soluble in DMSO...
15 July 2019 4,370 3 View
I am planning to order antibodies for my experiment which can be used for ChIP, Western blot, Immuno-fluorescence (IF) and Immuno-histochemistry. Some suppliers mention their product to be...
12 February 2018 5,006 4 View
Just curious if there can be somaclonal variation in direct organogenesis or not; because in direct organogenesis i.e. from explant to shoot and then root occurs directly without the intermediate...
05 August 2017 2,061 4 View
I talked with one of my professor; he told that chimeric plants are mostly found in T0 generation only and the opportunity for finding a chimeric T1 generation plant is extremely rare or most of...
12 June 2017 4,121 25 View
I am doing a 12% SDS PAGE gel after total plant protein extraction from cotton leaf by TCA/ACETONE precipitation method. I am loading the crude protein without dilution after keeping it for...
27 April 2017 786 21 View
I want a total plant protein extraction protocol specific for cotton leaf to do SDS-PAGE of the extracted total plant protein. what should be the pH of the extraction buffer? I want the proteins...
26 February 2017 7,717 3 View
What I have heard is the normal Taq Polymerase which we commonly use can amplify a fragment upto 1.5 kb. But yesterday someone told me that the normal Taq Pol can amplify a fragment upto 3kb...
22 September 2016 1,864 3 View
Generate XRD Pattern using Reitveld Refinement Paramenters
01 June 2016 2,134 6 View
This method of PCR amplification produces large concatamers of multi-looped amplicons those may show prominent visibility on the gel. But how this confers specificity of the amplification. Or if...
12 April 2016 2,469 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View