Hi all, I've been running electrophoresis gels for plasmid recombination but I'm having some difficulties with the ladder (TriDye). I am using a 1% agarose gel, with TAE 1X buffer and running it for 45min at 90V. However, the ladder looks distorted.
I have tried changing the batch of agarose and preparing fresh TAE each time, but I still have the same problem.
Ah, the bands look a little smeary, both for your ladder and your other samples. Try washing the comb with mild soap and warm water. Sometimes dried gel gets stuck on the comb and you end up with mishaped wells.
The jagged nature of the bands suggests that you are not dissolving the agarose completely. Heat for longer and swirl the agarose for longer before pouring the gel. look at a light through the gel and if you can see small, clear gel particles keep mixing until they dissolve
I agree with Paul Rutland that it seems like an agarose problem.
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