What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Native gel blot unable to to observe antibody binding?

Hello, I'm trying to utilize a native gel for a protein dimer experiment. Everything seemed to work relatively well until I try to examine the blot in a ChemiDoc MP. I don't see any antibody...

30 September 2020 6,059 3 View

Isolates that grow on CHROMagar col-APSE will not grow on MacConkey agar supplemented with Colistin Sulphate?

I prepare the CHROMagar Col-APSE (for selection of colistin resistant gram-negative bacteria) exactly to manufacturers guidelines. I have E. coli isolates that grow perfectly well on this media,...

26 March 2020 9,377 4 View

Can anyone recommend a proper procedure using mitomyocin C to inactivate mice T-cells in order to perform a proper mixed lymphocyte reaction?

This MLR will be done using two different strains of mice that is why it is necessary to inactivate the T-cells, so that possible proliferation can be observed.

06 October 2016 2,257 3 View

Can anyone help me with my a stent and balloon inflation model in Abaqus?

22 November 2015 6,911 15 View

Can transient transfection using COS-7 cells with a plasmid encoding a neomycin resistance be used a positive transfected cell selection method?

I want to do a transient transfection using COS-7 cells with a plasmid encoding a neomycin resistance, can I use this as a positive transfected cell selection method? COS-7 cells transfect with...

04 March 2015 9,231 5 View

Are cos-7 cells good for patch clamp?

I have been trying to patch transfected cos-7 cells (Kv channel plasmid) and am having an incredibly difficult time getting a seal let alone breaking in. Has anyone every patched with these cells...

10 December 2014 3,117 2 View

What do I do if my marginal effects are not significant in a probit model?

I have significant variables in the probit model but none in the marginal effects after using mfx in stata.

26 March 2014 7,811 2 View

How do I optimize PCR reactants?

Hi everyone! Attached you will a EtBR gel showing failed PCR results in lanes 2 and 3, with positive results for a DNA ladder in lane 1 (100 bases). Additionally, I have attached a photo of the...

01 January 1970 3,528 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View