DNA extraction from the leftover of protein extraction

07 June 2017 1 1K Report

Hi everyone,

I have been looking for a protocol to extract DNA from the leftover of protein extraction. I want to use the leftover of protein extraction (usually, the one left after we transfer supernatant-containing the proteins) -- usually, in the pellet kind of form.

Any suggestions? Looking forward to it. Thanks in advance.

Cheers ! Rajesh

Matthew Sobo

I'm not sure how well you'll get DNA out the back end of a protein prep--many protein preps don't get rid of the DNA. Every protocol I've ever seen has you pull out the DNA fraction then you can get protein out of its effluent.(Trizol or TRIreagent for instance).

That said, if you've already taken the protein fraction, you can try what Ali suggested.

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