Do you have experience with extraction DNA from lichens and myxomycetes?
I am using micro Plant extraction kit (Zymo), but success rate is very low. My starting material is very small...and I am fighting with elution step...I try incubation, incubation with Polymerase K, freezing in -80°C ant then disrupt it with pellets and put warm lysis buffer...I din't use beads with Magnalyser, because the material is so small... Do you have any advice?? THANKS IN ADVANCE!!
Dear Tatiana,
I think it will be useful to address your question to Yuri Novozhilov, here on Researchgate. His group had some problems with DNA extraction from myxomycetes but they managed to solve it at last. May be this link to their paper will be helpful too:
https://www.researchgate.net/publication/257694810_Phylogenetic_position_of_the_enigmatic_myxomycete_genus_Kelleromyxa_revealed_by_SSU_rDNA_sequences
Best wishes,
Elena
Article Phylogenetic position of the enigmatic myxomycete genus Kell...
Dear Alena,
thanks for recommendation and also for paper...I am following the similar workflow, except the ball mill...but I could try the Magnalyser..
Thanks! All the best,
Tatiana
- Badges
- Science method