DNA Extraction from Formalin Fixed Paraffin Embedded Tissue (FFPE) samples?

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Waleed Al Amri

Try commercial silica columns tube. I found they get you better gDNA yields from FFPE samples

Saliha Rizvi

there are various DNA extraction kits available in the market which would help you isolate DNA from FFPE samples like

1. QIAamp DNA FFPE Tissue Kit (QIAGEN)

2. FFPE DNA Extraction (Thermo Scientific) etc

Alfonso Saera-Vila

I agree with previous answers, commercial kits should do a very good job.

Good luck!

Byron Flores

The best way to extract DNA from paraffin blocks is using organic solvents such as xylene, heating and centrifuging, then wash with alcohol several times and finally with water several times, practically carry out the process in reverse, then proceed as indicate commercial kit

Elmara Graser

https://www.analytik-jena.de/en/life-science/products/prod/cat/isolation-of-genomic-dna/prod/blackprep-ffpe-dna-kit.html

One of our :-)

Rogelio Cruz

You have to remove first the tissue from the paraffin wax using xylene (clearing agent) then ( rehydrate ) place the section in decreasing alcohol concentration 95, 80, 70, 50, 40, 30, 20 and PBS, before extracting the DNA.