DNA extraction from formaldehyde preserved samples

More Francisco Kuhar's questions See All

Succesful large scale examples of biobased processes in paper or textile industry?

Are there experiences of bio based technologies as biopolish (textile industry), biopulping or biobleaching (paper industry). Hundreds of papers show promising results using fungal enzymes but it...

04 May 2019 2,644 2 View

What is the reason there is so much research on fungal laccases and only a few industrial suppliers?

What is the reason there is so much research on fungal laccases and only a few industrial suppliers? are they really used in the industry? or they have been replaced by something else? it seems...

01 February 2019 4,334 7 View

Does sequestration of heavy metals by the extracellular EPS matrix also count as uptake?

while working with a white-rot species we found that the (in vitro) retention of these elements was linked to the presence of guaiacol derivatives. However, we belive that heavy metals (among...

01 January 1970 4,014 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Zeena Fouad

Article Comparison of different methods for DNA extraction from form...

Aalaa Fahim Abbas

http://www.sciencedirect.com/science/article/pii/S0361090X0300103X

Francisco Kuhar

Thanks Zeena and Aalla! I'll take a look at the papers and see what I can do!

cheers

Francisco

Md. Shafiullah Parvej

Please update your final result here.

Nuha Allban

Hi, this pdf may help you