I'm trying to do a nucleic acid extraction from cacti. Has anyone worked with that? I'm facing problems to extract DNA and RNA from the aerial part, mainly due to the amount of mucilage. Most of the references about the subject do extract (D/R)NA from fruits, but I need to do the extraction very early after germination.
If any of you have worked with this and have a reference or any suggestion, it will be much appreciated.
Jai, thanks for your answer.
As a reference any cacti could work, I think the problem is with getting rid off the amounts of sap.
I'm working with a mexican endogenous plant, Pachycereus pringlei. There's no much articles published using that species.
http://www.actahort.org/books/995/995_6.htm
http://eduemojs.uem.br/ojs/index.php/ArqMudi/article/viewFile/20598/10923
http://www.scielo.br/scielo.php?pid=S0100-54052011000200007&script=sci_arttext
https://repositorio.ufsc.br/handle/123456789/79908
You may look for these papers:
http://hortsci.ashspublications.org/content/35/6/1124.full.pdf
http://www.bioone.org/doi/abs/10.3732/apps.1200013
Another possibility to avoid the problems with the mucilage is to extract the DNA from seeds or very young seedlings.
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