What should be the percentage of DMSO for dissolving crystal during MTT Assay ?

I am doing MTT assay. I have added 30ul of MTT to cell in 200ul in 96 well plate. After 3hr incubation, I need to dissolve violet color crystal, for that purpose how much percentage of DMSO will...

26 June 2020 2,881 2 View

How to mix essential oils with sublimated materials?

I want Mixing an aromatic oil with a sublimation materials that allows the oil to gradually penetrate for the longest period to control insect in lab condition

08 December 2019 6,649 1 View

How to perform Partitioning of variance to define the role of factors on insect development?

I have performed the experiment where I fed green tomato fruits (natural host) to the Helicoverpa armigera under six different alternating temperatures as treatments viz. 25:10, 25:13, 25:16,...

02 November 2018 4,126 2 View

Which is most preferred for calculating variances and standard error of life table parameters at different treatments? Bootstrap or jacknife?

Recently, I have analysed my results of life table parameters of an insect using jacknife procedure. I have also come across papers analysing same results using bootstrap. I want to know which...

30 August 2018 5,154 3 View

Is it right to mention transformed values of data, if the data has been analysed using Tukey's HSD post hoc?

I have analysed per cent survival data of an insect and also find out its arc sine transformed values. Is it appropriate to mention these values if I have analysed my data using Tukey's HSD post hoc?

27 July 2018 2,704 3 View

How to get equation for 3D scatter curve in spss?

I have worked upon impact of alternating temperature which is diurnal and nocturnal temperature on insect. I found that I can plot 3D scatter curve but how to get its equation in spss 23 version...

05 March 2018 9,143 1 View

What is temperature pattern in incubators-Sine?, Ramp?, Square?

I carried out an experiment related to impact of alternating temperature on growth of Helicoverpa armigera insect under growth chamber by changing the temperature after 14 and 10 hrs. Whether...

01 February 2018 1,102 2 View

How to feed expression of non linear lactin model in SPSS?

I am facing problem with feeding expression given in the picture to determine maximum thresold temperature

19 September 2017 5,494 1 View

Why 100 per cent sterilization is not reported in lepidopterous insects by using sterile insect technique?

12 September 2016 1,897 15 View

Which insecticide or fumigant is used during fogging operations by municipal corporations in India against mosquito menace?

Time and again I have been reading about spike in dengue and chikunguniya cases and measures taken by municipal authorities in the form of fogging for controlling responsible factor like mosquito....

11 September 2016 2,257 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View