EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can I performe behavioral tests in the same rats at PND30 and PND60?

Hi! Anyone knows whether I can performe behavioral tests in rats at PND30 and then, repeate them at PND60? Or this is not correct? Thanks.

20 July 2020 3,659 3 View

Load-Displacement curves with XFEM in Abaqus?

Hi everyone, I am working with abaqus by using XFEM. I am modelling a Single Lap Joint and I have simulated the crack propagation within the adhesive. Nonetheless, I also want to extract the...

18 March 2020 7,688 5 View

Anyone know the scale of tolerance of skin cells in culture, to ethanol?

I am working with cell culture and using ethanolic extracts of plants, I would like to know what percentage of ethanol from which influences the viability of the cells.

24 January 2019 8,924 3 View

DNA extraction kit for metagenomics?

Hello, I would apreciate your knowledge and answers :) I pretend to extract DNA of leaves of a plant with a lot of contaminants ! I tried before and i couldnt, even with purification! Now, i am...

24 January 2017 4,716 3 View

Phylogenetic analysis of fastq files from NGS (ion torrent) amplicon sequencing?

06 May 2015 4,487 3 View

What is a software for phylogenetic analysis (SNPs) to use on fastq files?

Hi, I want to analyse fastq files from DNA amplicon sequencing and make a phylogenetic analysis of SNPs. Can someone indicate me a good (easy?) software to do that? thanks

30 April 2015 1,566 3 View

How to prove that water circulates in hollow part in wicking test of yarns?

anyone has any idea to prove, in wicking test of yarns, that water circulates in hollow part besides to circulate in the inter filaments space in hollow multifilament yarns?

18 February 2015 6,543 1 View

How can i do a regression with categorical variable no binary?

Hello, i tried using the binary logistic regression in spss,the following error message is being shown Warnings The dependent variable has more than two non-missing values. For logistic...

28 August 2014 640 23 View

FACS of Trypanosoma brucei parasites?

I am interested in sort blood stream forms (BSFs) of Trypanosoma brucei. So far I've been successful sorting the procyclic form cells of this parasite using a protocol that has been published...

02 December 2013 6,462 13 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View