How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

After G actin polymerization and labelling I didn't got filaments. Why?

Purified G actin and got 42kda band and an additional band just below it. While performing G actin polymerization and labeling with rhodamine-phalloidin I didn't observe filaments under the...

20 January 2021 3,751 2 View

Is it possible to use someone else's MOCAP data?

In order to study bone fracture in patients with osteoporosis, I need patient's exact muscle forces magnitude, location and direction (as FEM boundry conditions), while it is difficult to get...

18 September 2020 6,804 3 View

Can muscle force obtained from EMG while walking be validated with force obtained from CMC in opensim?

Can force obtained from EMG be compared with force obtained from computed muscle control in opensim?

17 April 2020 938 1 View

Is there any free (or student version) ground reaction forces prediction software that use motion data in input and evaluate 3-D force components?

I'm writing my degree thesis on the biomechanics of running using Opensim. I only could use monoaxial force plates, but the software needs all three components of force.

10 November 2017 5,141 0 View

How can a CAD model be implemented on OpenSim software?

Is there any stablished plug-in already developed to use over OpenSim for this purpose?. I wanted to do simulations with exoskeletons CAD designs to see the musculoskeletal behaviour. Thanks.

01 February 2017 9,014 3 View

Can someone suggest me step-by-step tutorials for OpenSim software (developed by Stanford University)?

I am presently using OpenSim software for biomedical simulation purposes. I need help in using this software. Also, anyone using this software kindly contact me at the earliest. Any other...

08 September 2016 8,568 0 View

Rift glasses and OpenSim?

Suggestions of papers and softwares to use rift glasses at virtual world OpenSim?

05 June 2016 624 3 View

I run opensim- matlab for arm, but error in matlab "block error" ,how i correct it?

I use S function in matlab ,it get opensim file but error in initial callback, how i check in model properties initial callback?

03 February 2016 4,737 1 View

I run opensim- matlab for arm, but error in matlab "block error" ,how i correct it?

I use S function in matlab ,it get opensim file but error in initial callback, how i check in model properties initial callback?

01 February 2016 3,902 1 View

I'm looking for OpenSim gait model for transfemoral, is any one know?

I'm using OpenSim to simulate gait for healthy subjects, I would like to simulate gait data for transfemoral amputee. thanks

31 December 2015 2,427 0 View

Do u know about opensime software?

11 December 2015 8,733 0 View