How can I optimize the pH of biosynthesis AgNP? Is it acceptable to use chemical reagents to adjust the pH in green synthesis approaches?

More Michelle Darmawan's questions See All

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

When is it optimal to choose an OFAT (One factor at a time) design over DOE (design of experiment)?

As a current undergraduate student majoring in Microbiology who is still learning DOE (Design of experiment) on my own, I have heard and read that an OFAT design is considered the most inefficient...

29 January 2021 8,256 3 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Why is my blot transfer bad now?

I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...

23 December 2020 670 3 View

How/where to provide a dataset?

If someone has collected a large useful dataset that could be useful to social science research, gender research, a specific industry sector in business, and possibly business students or those...

05 October 2020 9,236 6 View

Does amelanotic B16-F10 cells give rise to black toumour nodles when injected in mice?

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02 September 2020 3,524 1 View

Why do 293T cells starts dying after lentiviral transfection?

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16 August 2020 6,647 3 View

Any ideas on where to look to for organization theory that speaks to why people join organizations?

I am studying place attachment. Place attachment is usually examined by looking at place and the emotional connection individuals have with place. However, I am interested in learning about how...

11 June 2020 9,483 7 View

What are some of your favorite mixed-methods studies related to environmental management and citizen science/stewardship?

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25 May 2020 4,994 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

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02 March 2021 2,735 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Ester cleavage conditions?

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01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

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01 March 2021 4,306 1 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

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01 March 2021 8,359 1 View

Which bacteria are suitable for quorum sensing screening?

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01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Yuri Mirgorod

Michelle Darmawan

Use a green extract pH of 7-8. Adding alkali or acid may cause the extract components to decompose.

Thank you for the advice Sir Yuri Mirgorod . If I may ask, how can I prepare my plant extract to achieve a pH of 7-8 without adding acid or base?

You can try to achieve this pH by increasing the temperature, pressure, or adding another extract.

Thank you very much for the explanation and guidance, sir Yuri Mirgorod. I will try to implement it.