I have studied the antioxidant activity of my three samples (tungsten disulfide) prepared solvothermal by changing the solvent alone. It seems that % inhibition is more for the sample2, but its ic50 value is higher than sample3 which has less % inhibition than sample2. What is the reason behind this discrepancy?
Dear Mr. Vishnu Sankar
Let us simplify: S2 = sample 2; S3 = Sample 3
Do you mean that S2 > S3 for % inhibition, and
S2 > S3 for IC50?
What % inhibition and IC50 assay did you use?
That could be a different mechanism of the tested compound. The antimicrobial agent could work by inhibiting cell wall synthesis, depolarizing the cell membrane, inhibiting protein and nucleic acid synthesis, and inhibiting or blocking metabolic pathways in bacteria. Otherwise, the mechanism of antioxidant activity using the DPPH assay is by scavenging the DPPH radical. Your tested compound may have a high capacity to scavenge the DPPH radical, but it has not any activities to kill microbes.
Actually, I am still confused about your question because the results of the DPPH assay can be presented by both % inhibition and IC50.
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