Direct injection, CID, 70 V: standard. UPLC-separated, DDA, 90 V: in-brain-sample analyte. Same MS/MS profiles. Thoughts? Experiences?

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How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

In SPSS, how to select cases if variable has x but not y?

Hi all, I have an SPSS longitudinal database in which one of the variables is monthnumber. The month ranges from 0 (baseline) through all of the months they were involved and then 51 for last...

30 November 2020 490 4 View

What is the best way to analyse rainfall data against a second variable?

I am looking at how rainfall can influence volcanic activity. I have rainfall data from three different datasets. One is a gridded dataset at 0.25 x 0.25 degree resolution that offers monthly...

04 November 2020 5,970 3 View

Two peaks in an antibody purification through AKTA purifier?

When purifying monoclonal antibodies from hybridome cultures (protein G affinity chromatography) we got two different peaks, which we collected separately. When analysed by SDS-PAGE they both...

06 September 2020 3,662 5 View

How do I merge variables in SPSS?

I merged two datasets with different cases; one dataset has two cohorts/comparison groups and the other dataset has one cohort, the experimental group. Some of the variables are named the same and...

19 August 2020 5,150 3 View

Can I still identify a specific cell type by flow cytometry if the cells are dead or do I need to pre-sort to study cell death of a given population?

Hi everyone! I want to study the response of plasma cells to different drugs after in vitro exposure. Can I culture whole bone marrow (after RBC lysis), treat with my drug, wait an appropriate...

03 August 2020 8,194 6 View

Is is viable to conduct a content analysis on social media posts?

I'm intending on conducting a content analysis on the stigmatising effects of a recent anti-obesity campaign. I would like to do this analysing the comments in response to Instagram posts by a...

06 April 2020 5,341 10 View

Cable stayed bridge cable to deck connection and column?

What is a suitable connection and any good websites to explain the connection of the cables to the deck surface and the cables to the column on a single column cable stayed bridge.

06 December 2019 3,244 2 View

How to detect the mechanical property of powders by nanoindentation?

Hi everyone. Does anyone know how to hold the powders (10um) when doing nanoindentation? I have mixed the powders with epoxy, and let it dry, then polished the mixture, but I never got converged...

14 October 2019 3,100 5 View

Architectural Engineering research topic that links with interior design?

Looking for a topic for my dissertation that could link architectural engineering and interior design together. I'm thinking of studying interior design after architectural engineering and would...

18 August 2019 6,993 10 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View