Direct injection, CID, 70 V: standard. UPLC-separated, DDA, 90 V: in-brain-sample analyte. Same MS/MS profiles. Thoughts? Experiences?

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Amy N W Schnelle

John, I appreciate your recommendation. In this case, I am confident in my results. An additional study to use as a reference for my manuscript is the purpose of this question.

Navid J. Ayon

I think its indeed a matrix effect. Did you run LC MS to check? You might require some sample clean up.