Dimer noticed instead of monomer on western blot?

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Hi,

i performed western blot to determine the protein expression. I expect a band at 22 kDa, interestingly the strong protein band noticed as dimer at 44 kDa. May i know what could be the reason?

TIA

Greg A Kirchenbaum

Did you reduce the sample with B-ME or DTT prior to running the SDS-PAGE?

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