Hello everyone, can you explain me how you do the dilution of the samples (extracts) in the ABTS and FRAP assay?
What solvent do you use and what is the blank for each assay to read the absorbance at 734 and 593 nm.
Thank you for the information
Melissa García Reyes
What is the nature of extract.(i.e. Polar or non polar).
If polar you can dilute the samples in deionized water and also prepare Stock ABTS and pottasium per sulphate in same water.
If sample mid polar, after preparation of stock reagents u dissolved in ethanol or acetone.
Thank you :D and is non polar. A question more, what is the blank for the FRAP Assay?, to calibrate to zero, water, methanol or DMSO? :/ or What is the absorbance of FRAP solution?, because it´s the absorbance control.
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