Tolerogenic Dendritic cells - in vivo functional assessment in animal models?

The function of human monocyte-derived DCs (either tolerogenic or immunogenic) is most frequently studied by various in vitro assays (e.g. MLR, Treg suppression assay, CTL induction, etc.) Does...

08 September 2019 8,725 1 View

Can you really detect necrosis using Annexin/PI or Annexin/7-AAD kits??

A lot of companies are marketing their Annexin/PI or Annexin/7-AAD kits as apoptosis/necrosis detection kits. However, can you really use these kits to separate between late stage apoptotic...

31 December 2015 825 4 View

What is the maximum number of pure (95%) CD4+CD45RA+ naive T cells from one buffy coat?

Isolation of CD4+CD45RA+ T cells with high purity is essential for immune functional assays. Can you please share your experience with isolation of CD4+CD45RA+ (using Miltenyi isolation kits),...

11 December 2015 9,042 6 View

NBF grant cycle 2015 Application - Does anybody have an older sample of an application??

Dear all, I intend to apply for 2015 NBF grant cycle (AABB). Since this is my first time, some things on their templates do not seem straightforward and I would really benefit in time if I had an...

11 December 2014 1,084 0 View

Which is the best of the different sources for mouse CD4+ T cells, spleen or lymph?

Can anyone explain, which source is best for isolation of mouse CD4+ T cells for basic functional assays, spleen or lymph nodes? In addition, I haven't noticed anyone isolating CD4+ T cells from...

11 December 2014 2,766 5 View

Does anyone have information where to purchase mice with progeria?

Looking for mouse models with progeria. Does anyone know where to purchase them?

07 August 2013 2,775 5 View

What are the highest local IFN-gamma levels that can be produced during an in vivo response?

Does anyone have any information on the max levels of IFN-gamma that can be produced locally during various in vivo responses?

10 November 2012 8,631 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View