Does anyone have any information on the max levels of IFN-gamma that can be produced locally during various in vivo responses?
hey Urban, we did not do local but can tell you in the blood. After trauma/hemorrhage hit it peaked around 2ng/ml (24h post by a FACS-based bead assay) and in cecal ligation and puncture in mice (simulates human abdominal sepsis) IFNg peaked at around 0.52ng/ml in late sepsis (days 6-28 post-CLP) and 1.6ng/ml in early (days 1-4 post-CLP) sepsis (all female CD-1 mice).
Hope this helps a bit. Best of luck with your research! Martin
hey Urban, we did not do local but can tell you in the blood. After trauma/hemorrhage hit it peaked around 2ng/ml (24h post by a FACS-based bead assay) and in cecal ligation and puncture in mice (simulates human abdominal sepsis) IFNg peaked at around 0.52ng/ml in late sepsis (days 6-28 post-CLP) and 1.6ng/ml in early (days 1-4 post-CLP) sepsis (all female CD-1 mice).
Hope this helps a bit. Best of luck with your research! Martin
It depends on the location. For example, in patients with newly diagnosed pulmonary tuberculosis the mean value of IFN-gamma levels were found as to 9.3 +/- 4.6 pg/mL, whereas 9.8 +/- 3.8 pg/mL in patients with inactive tuberculosis, and 10.2 +/- 3.4 pg/mL in healthy controls.
See reference: http://www.ncbi.nlm.nih.gov/pubmed/16615013
Hi Urban. It depends on the model: mouse or human, and the factor inducing the IFNg response. During acute response you will find plenty of cytokines in serum or plasma, probably safer to test diluted samples. Some of the cytokines will be over the upper threshold of the assay (there's so much produced!). But also keep in mind that the active half life of cytokines is in the range of 30 sec to 2 min, the process of secretion/absortion is very rapid and they're quickly removed from circulation. IFNg is one of the most stable in circulation (in tissue culture too).
Thank you all so far! I am really interested how much IFNg could be found in a local acute reponse theoretically. I believe I once came across a publication mentioning that during an acute response, as much as 35 000 U/ml of IFNg can be produced in the microenvironment. I cannot remeber where I read this sadly...
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology