We sequenced 20 samples of 2 different regions. A total of 40 samples. We used different primer pairs aiming to recover a broad range of groups. However, PCR/sequencing failed for different primers and samples. Now I don't have any sample sequenced with all primers. For a 12S I have 4 samples from one area and 20 from the other. I know it is far from ideal, but I can't re-sequence and wouldn't like to lose the produced data. It's the first baseline from areas in Amazon. I don't have any more ideas on how to compare the two areas with such a big sample number difference.
Can someone help me?
Katie A S Burnette
The answer is you present the data and do not compare them. It's now a descriptive study with future direction of having enough samples to compare
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