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Basically I'm helping with the matrix using a cosmolitan group, could one of the characters used for be biogeographical regions (Neartic, Neotropical, etc)? Most of the data in this matrix refers...
25 July 2024 9,753 2 View
Hello, I'm working on medical project and I would need synchronized angiography with ECG? Does anyone know if some open source dataset of this kind exist? Regards, Bruno
25 July 2024 2,214 2 View
Hello all, I'm starting a project where we want to automate the video analysis of people working in different environments to produce ergonomic measures like hip flexion, shoulder extension, etc....
16 June 2024 1,843 1 View
Hello, I am making lipid nanoparticles using the thin film hydration method. Formula is DODMA:DSPC:Cholesterol:DSG-PEG2000 at 60:20:17.5:2.5 %mM in 5mM total. After hydration in sodium acetate...
16 June 2024 6,842 6 View
Hello, Im trying to make a nanoliposome to encapsulate plasmid DNA. I am using the standard thin film method followed by resuspension in sodium acetate 25mM pH4 with my DNA (Im using the...
05 June 2024 3,646 3 View
I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...
29 April 2024 8,231 1 View
Sid has retired from Clemson University, thus his address [email protected] is no more working,
28 April 2024 4,340 7 View
I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...
16 April 2024 2,051 2 View
I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation
06 April 2024 1,926 2 View
In alkaline solution (NaOH 0,1 M), methyldopa generates orangish solution. I suspect it turns into indolic compounds by base catalysed oxidation (possibly oligomers). How can I confirm...
01 April 2024 8,298 0 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View
i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...
30 July 2024 2,349 1 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
14 July 2024 641 1 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
It is well known that the larger the organism, the less energy consumed per unit mass (Delong et al. 2010; Moses et al. 2016; Wells 2007). This is known as Kleiber’s Law, whereby the energy...
06 July 2024 8,736 0 View
Hello everyone, I'm in the initial stages of conducting a study involving the ddPCR (Bio-Rad QX200, QX600) and I ran into some problems involving recovery % calculations using direct...
02 July 2024 6,011 2 View
