I am working with mouse embryonic fibroblasts which were immortalized with sv40 and were genetically modified (gene taggeing , transgene etc) and I would like to differentiate them to neural stem cells. did anyone had an experience with something similar? all the literature is usually having protocols for differentiating Primary cells- for example, using media with bFGF and EGF to help proliferating the cells but I wonder ..as they dont have a Problem to proliferate if I at all Need this. would be happy for any Input on the issue.